Altuve, who singled on the first pitch of the ball game Friday, said he was injured in the first inning when attempting to steal second base. He got the steal, but said he felt the soreness on the turn to second.
Altuve took one more at-bat to test the muscle, but decided to play it safe.
"It was when I tried to take second base, I just turned really hard, so that was bad of me," Altuve said. "After I [stole] that base, I felt really sore. I took the next at-bat, so when I swung it was a little worse."
Matt Downs, who was filling in at third base, made the move to second base, and Matt Dominguez, who had been out of the lineup nursing a wrist injury, came in at third.
Altuve was left out of Saturday's lineup and Astros manager Tony DeFrancesco said Altuve would be out "a few days."
"It's kind of deep up in his groin, so we're just going to have to wait and see how that goes," DeFrancesco said. "He has no activity [Saturday]. He did his treatment."
Altuve hopes to return to the starting lineup at some point next week when the team returns to Houston.
"I think it's day-by-day," Altuve said. "We'll see how it feels when I get home. We'll see if I can play."